ML phylogenetic tree of Physarales isolates based on the combined SSU/LSU data set. The analysis was based on the alignment of 2522 nucleotide positions from 33 isolates representing the Didymiaceae and Physaraceae families as well as Stemonitis flavogenita (order Stemonitales) as out-group. The evolution model is GTR + I + G. Tree reconstruction using NJ, MP, ML, or BAY resulted in essential identical tree topologies. Bootstrap values (> 50%) of 2000 replications are shown above the branches (NJ/MP/ML). Node of high statistical support (bootstrap values of 100%) is indicated by red circle. BAY analysis consists of 1,000,000 generations with trees sampled every 100 generations. BAY posterior probabilities are shown below branches, and branches with posterior probabilities of 1.0 are presented as thick black lines. The Physarales clades (Clades I to III) are indicated.