Comparisons of DNA methylation of 4 DMRs in PFC of multiple species. For each DMR, the orthologous regions (annotated by UCSC pairwise genome alignment) of 4 species were compared. DNA methylation levels were obtained by bisulfite clone sequencing, and the comparisons for each DMR are shown in A-D respectively. There were missing of Chimpanzee2 due to failed PCRs. The sequenced samples for human and rhesus macaque were H1 and M1. For RNF32 (D), there is no orthologous gene in the rat genome. The error bars represent the standard errors of the methylation levels of all the CpG sites of a gene.