Figure 5
From: Evidence for a divergence in function between two glucocorticoid receptors from a basal teleost
(A) Transactivation EC50 values for Pantodon buchholzi GR1 (black bars) and GR1-s (white bars). Note values for Pantodon buchholzi GR1 are taken form Table 1 (N = 3, average + SEM) and that GR1-s lacks the 21 amino acids at the C-terminal of GR1 (see Additional file 1 Figure S1). (B) Typical Scatchard plots for Pantodon buchholzi GR1 (black circles) and GR1-s (white circle). B, bound [3 H]-dexamethasone; B/U, ration of bound to unbound [3 H]-dexamethasone. The inset table provides the average binding affinities, Kd (nM) and maximum binding per well (nmol/well) for maximum binding per well for each receptor, values represent average (SEM) from 3 – 5 separate experiments.