Figure 6

AKAP7 Localization in rat and mouse heart. Merged immunofluorescence images of (A) adult rat and (B) mouse hearts were taken using a confocal microscope. Immunostaining was performed using a rabbit anti-AKAP7 antibody, the 2˚ Ab is Alexa fluor 488 anti-rabbit. Nuclei are stained with Dapi (blue). AKAP7 immunostaining is prominent in the sarcoplasmic reticulum in both rat (expressing AKAP7δ only) and mouse (predominantly AKAP7γ but also AKAP7δ). In the negative controls for rat (C) and mouse (D) normal rabbit serum (AKAP7 pre-immune serum) was used in place of the AKAP7 primary antibody. Images were taken at 60X magnification.