Neighbor-Joining phylogeny of PWWP containing proteins in the Sea Anemone Nematostella vectensis and in humans. The evolutionary history was inferred using the Neighbor-Joining method. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (5000 replicates) are shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. All positions containing gaps and missing data were eliminated from the dataset (Complete deletion option). Phylogenetic analyses were conducted in MEGA4. The full-size protein products of the three genes from the NSD (WHSC) family are included in the analysis. Each of the three proteins appears twice (annotated with an ‘a’ or ‘b’ proteins) in two separate clades on the phylogenetic tree, as the two PWWP domains of each protein form separate clusters (see text for details). The distinct PWWP-domain subgroups (as discussed in the main text) are shaded in different colors. Species abbreviations correspond to Nematostella vectensis (Nv) and the rest of the proteins correspond to humans.