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Figure 5 | BMC Evolutionary Biology

Figure 5

From: Sponge non-metastatic Group I Nme gene/protein - structure and function is conserved from sponges to humans

Figure 5

Subcellular localization of NmeGp1Sd and human Nme1. A) HEp-2 cells transiently transfected with pEGFPN1-Nme1 (green fluorescence) and pDsRed-NmeGp1Sd (red fluorescence). The signal is visible mainly in the cytoplasm. The overlay (yellow) shows colocalization of the human and sponge homologs. B) HEp-2 cells transiently transfected with pEGFPN1-Nme1 (green fluorescence) and pDsRed-NmeGp1Sd (red fluorescence). The signal is visible mainly in the cytoplasm. The "granum-like" structures are visible in the cytoplasm, and they are formed with both, the human and the sponge homolog. The overlay (yellow) displays the subcellular colocalization of human and sponge homologs. C) HEp-2 cells transiently transfected with pEGFPN1-Nme1. The signal is present in the cytoplasm, but also in the nucleus, although not so prominent. D) HEp-2 cells transiently transfected with pEGFPN1-NmeGp1Sd. The signal is present in the cytoplasm and in the nucleus. Bar = 10 μm.

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