Table 3 Plasmid standard curve values
Plasmid::gene of interest | Linear Equation | Amplification Efficiency (%) | r-squared values (Rsq) |
|---|---|---|---|
Primary survey | |||
A180 | Y = -4.976*Log(X) + 56.76 | 58.8 | 0.979 |
P180 | Y = -4.539*Log(X) + 51.69 | 66.1 | 0.981 |
S180r | Y = -3.494*Log(X) + 43.42 | 83.8 | 0.993 |
S180 | Y = -3.998*Log(X) + 51.22 | 77.9 | 0.986 |
RH2-1 | Y = -4.141*Log(X) + 52.61 | 74.4 | 0.995 |
RH2-2 | Y = -3.846*Log(X) + 41.51 | 82.0 | 0.996 |
SWS2A | Y = -3.881*Log(X) + 43.15 | 81.0 | 0.942 |
SWS2B | Y = -4.142*Log(X) + 49.30 | 74.4 | 0.993 |
SWS1 | Y = -3.378*Log(X) + 38.49 | 97.7 | 0.998 |
RH1 | Y = -4.036*Log(X) + 39.05 | 76.9 | 0.990 |
COI | Y = -3.828*Log(X) + 41.05 | 82.5 | 0.991 |
B-actin | Y = -3.402*Log(X) + 38.67 | 96.8 | 0.996 |
Myosin-HC | Y = -3.651*Log(X) + 39.80 | 87.9 | 0.999 |
Secondary survey | |||
COI | Y = -4.132*Log(X) + 47.75 | 74.6 | 0.957 |
B-actin | Y = -3.861*Log(X) + 42.84 | 81.6 | 0.973 |
Myosin-HC | Y = -4.217*Log(X) + 50.74 | 72.6 | 0.988 |
A180 | Y = -3.774*Log(X) + 43.27 | 84.1 | 0.998 |
S180 | Y = -4.184*Log(X) + 51.10 | 73.4 | 0.984 |