Skip to main content
Figure 1 | BMC Evolutionary Biology

Figure 1

From: RT-qPCR reveals opsin gene upregulation associated with age and sex in guppies (Poecilia reticulata) - a species with color-based sexual selection and 11 visual-opsin genes

Figure 1

Fold expression of guppy visual opsin genes in juvenile and adult eyes. One- and two-month old juvenile (1MOJ and 2MOJ, respectively) and adult (male and female) cDNA samples are derived from left (L) and right (R) eye mRNA pools isolated from 6-12 individuals for each age or sex (see Table 1). Opsin transcript copy number was normalized to the transcript copy number (geometric mean, represented by dashed line) of three reference genes: COI, β-actin, and Myosin-HC (asterisk). Error bars (±S.E.M. of triplicate reactions) are shown for all samples. Colors represent the λmax of each opsin (based on Wavelength to RGB v.1.0 software), excluding RH1.

Back to article page