Figure 1From: Functional conservation of the Drosophila hybrid incompatibility gene LhrHP1 immunoprecipitates with and binds directly to LHR. A) HP1 immunoprecipitates with LHR in extracts from Drosophila Kc embryonic tissue culture cells. Anti-MYC immunoprecipitates from wild-type Kc cells (control) and LHR-MYC expressing cells were analyzed by Western blot using anti-MYC and anti-HP1 antibodies. The percent of each sample loaded is shown (%). Protein that did not immunoprecipitate is shown in the flow through lane (FT). B) LHR binds to HP1 in an in vitro binding assay. In vitro synthesized LHR-HA and HP1 proteins were mixed and the resulting complexes were immunoprecipitated with anti-HA antibody and analyzed by Western blot using anti-HA and anti-HP1 antibodies. Control lane is sample immunoprecipitated from an in vitro synthesis extract without DNA from either gene added.Back to article page