Comparison of Pla and Epo. Pla and Epo were compared in (A) plasminogen activation, (B) degradation of plasminogen, and (C) degradation of α2-antiplasmin. pla and epo were expressed in recombinant E. coli XL1. The strains are indicated with their plasmid names (see Table 1). In (A), plasmin formation was measured using a chromogenic plasmin substrate. The data are average of two independent assays with duplicate samples, and standard deviations are shown. In (B), Western blotting with anti-plasminogen (top panel) and anti-plasminogen catalytic domain (bottom panel) antibodies is shown. The black arrowhead indicates plasminogen and the white arrowheads indicate cleaved plasminogen, the heavy chain (top panel) and the light chain (bottom panel) of plasmin. In (C), degradation of α2AP was visualized with Western blotting using α2AP antibody, and the black arrowhead indicates uncleaved α2AP and the white arrowhead indicates the degradation product.