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Figure 6 | BMC Evolutionary Biology

Figure 6

From: Fused eco29kIR- and Mgenes coding for a fully functional hybrid polypeptide as a model of molecular evolution of restriction-modification systems

Figure 6

Analysis of the AdoMet effect on REase activity of RM.Eco29kI. A - reactions were carried out in REase optimal buffer in the absence (left panel, -AdoMet) and presence (right panel, +AdoMet) of AdoMet; B - reactions were carried out in MTase optimal buffer in the absence (left panel, -AdoMet) and presence (right panel, +AdoMet) of AdoMet. Triangles above gels mark 2-fold dilutions of the enzyme in the reaction mixtures. The figure represents ratio of REase and MTase activities in RM.Eco29kI enzyme.

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BMC Ecology and Evolution

ISSN: 2730-7182

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