Figure 2

Sliding window plot and motifs comparison of MEPE across the 26 mammalian species. Sliding window plot of GC-content and nucleotide and amino acid conservation among the 26 mammalian MEPE coding sequences (exons 2, 3 and 4) that were used in this evolutionary study. The plot was calculated after pairwise deletion of ambiguous sites and the windows were adjusted to correspond to the same scale. The blue shading identifies conserved regions (> 80% nucleotide or amino acid similarity), the red line tracks nucleotide similarity, the green line amino acid similarity and the black line % GC content. The three motifs/regions are represented within the boxes A-Signal Peptide-, B-Dentonin (SDGG, RGD), C- Putative regulatory regions and D-ASARM. The yellow and red shadow represents selection at codon level and amino acid level, respectively, while the grey shadow correspond to the species excluded from the positive selection analyses at site level.