Figure 2

Atypical nucleotide composition. (A) GC content anomaly. GC content anomaly. Percentage of total GC content and GC content at codon positions of all L. major ORFs and L. major META1. The vertical error bars represent the mean value +/- 2 S.D. for L. major genome. Total, total GC content; GC₁, GC content at first codon position; GC₂, GC content at second codon position and GC₃, GC content at third codon position. (B) Codon usage bias. Codon Adaptation Index (CAI) of 7 reference genes (Amastin, GP63, Hexokinase, Ribosomal Protein (RP) S8, α-Tubulin, PTR-1, Coproporphyrinogen (Coprogen)) and META1. The accession numbers of the protein sequences used are given in Additional file 2.