Cis-regulatory activity of the 2D3.1 and 4UP2.1 deletion constructs. A) Schematic representation of 2D3.1 deleted fragments and of their expression in Ciona embryos at tailbud and larva stages. B) Construct 2D0.5 is active in the palps and sensory vesicle, as for construct 2D3.1, and also in the caudal sensory neurons (C), in the pharynx and visceral ganglion (D). E) Summary of 4UP2.1 deletion constructs and of their activity in transgenic Ciona embryos at tailbud and larva stages. F, G) 4UP 0.8 construct is expressed in the anterior CNS at tailbud stage (F) and in the mesenchymal pockets at both tailbud and larva stages (F, G). E, EcoRI; H, HindIII; RV, EcoRV; X, XbaI.