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Figure 3 | BMC Evolutionary Biology

Figure 3

From: Molecular characterization and evolution of a gene family encoding male-specific reproductive proteins in the African malaria vector Anopheles gambiae

Figure 3

FISH mapping of the genes on polytene chromosome of A. gambiae, A. arabiensis and A. merus. FISH was performed with a probe specific to AgAcp34A-1 and a probe common to AgAcp34A-2 and AgAcp34A-3 on polytene chromosomes of A. gambiae (top panel), A. arabiensis (middle panel), and A. merus (bottom panel). Overlapping red and blue signals on chromosomes counterstained with YOYO-1 are shown on merged images (a, d, g). The AgAcp34A-2/3 probe is labeled with Cy3-AP3-dUTP (red signal in b, e, h). The AgAcp34A-1 probe is labeled with Cy5-AP3-dUTP (blue signal in c, f, i). Arrows indicate the site of localizations of the blue and red signals in subdivision 34A of the 3R arm.

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