Physical mapping with ASGR-linked BACs on chromosome spreads from various Pennisetum and Cenchrus species. a-j, Color-merged images of FISH signals and inverted DAPI-stained chromosomes; red and green arrows indicate P001 and P208, respectively; insets show enlarged, pseudo-colored ASGR-carrier chromosome. k-m, Images of dual-labeled FISH on DAPI-stained chromosomes. a, m: P. mezianum (PS9); b: P. orientale (PS12); c: C. setigerus (PS16); d: P. polystachion (PS19); e: P. setaceum (PS25); f: P. flaccidum (PS95); g: P. subangustum (PS163); h: P. villosum (PS249); i: P. pedicillatum (PS304); j: P. massaicum; k: P. massaicum spread in panel j stripped and rehybridized with rDNA and P602 (red); green arrows and signals indicate rDNA; outlined chromosomes did not hybridize with P602. l: C. setigerus hybridized with rDNA and P208; green signals are rDNA and red arrow indicates P208 signal. m: P. mezianum (PS9) hybridized with P208 (red arrow) and P602 (green signal). Bars correspond to 10 μm.