Skip to main content

Table 2 Primers for PCR

From: Loss of genes for DNA recombination and repair in the reductive genome evolution of thioautotrophic symbionts of Calyptogena clams

Primer Nucleotide sequence Target gene Coding amino acid sequence Note
recA Fi 5'-GAYGCNGARCAYGCIYTNGAYCC-3' recA DAEHALD ip
recA Ri 5'-ACNCCDATYTTCATNCKDATYTG-3' recA QIRMKIGV ip
recA_F 5'-GATTGCATATCATTCATCTGATAACG-3' recA non ep
recA_R 5'-AGTGGATTRGGATCAAGCATAGC-3' recA non ep
mutY_F 5'-CCTATATGWACACCATCAAAGTTGCC-3' mutY non ep
mutY_R 5'-AATACTTGGRGWAACGCCACTAATG-3' mutY non ep
27F 5'-AGAGTTTGATCCTGGCTCAG-3' 16S non ip
1492R 5'-GGTTACCTTGTTACGACTT-3' 16S non ip
23S F1 5'-GGGAACTGAAACATCTAAGTACC-3' 23S non ip
23S R1 5'-CCCGCTTAGATGCTTTCAG-3' 23S non ip
  1. ip, internal primer; ep, external primer.
  2. 16S, small subunit rRNA gene; 23S, large subunit rRNA gene.