Table 2 Primers for PCR
Primer | Nucleotide sequence | Target gene | Coding amino acid sequence | Note |
|---|---|---|---|---|
recA Fi | 5'-GAYGCNGARCAYGCIYTNGAYCC-3' | recA | DAEHALD | ip |
recA Ri | 5'-ACNCCDATYTTCATNCKDATYTG-3' | recA | QIRMKIGV | ip |
recA_F | 5'-GATTGCATATCATTCATCTGATAACG-3' | recA | non | ep |
recA_R | 5'-AGTGGATTRGGATCAAGCATAGC-3' | recA | non | ep |
mutY_F | 5'-CCTATATGWACACCATCAAAGTTGCC-3' | mutY | non | ep |
mutY_R | 5'-AATACTTGGRGWAACGCCACTAATG-3' | mutY | non | ep |
27F | 5'-AGAGTTTGATCCTGGCTCAG-3' | 16S | non | ip |
1492R | 5'-GGTTACCTTGTTACGACTT-3' | 16S | non | ip |
23S F1 | 5'-GGGAACTGAAACATCTAAGTACC-3' | 23S | non | ip |
23S R1 | 5'-CCCGCTTAGATGCTTTCAG-3' | 23S | non | ip |