Table 2 Primers used in this study
From: Schmidtea mediterraneaphylogeography: an old species surviving on a few Mediterranean islands?
Name | Sequence 5'-3' | Annealing Temperature (ºC) | Source |
|---|---|---|---|
COI | |||
COIF (F) | CCNGGDTTTGGDATDRTWTCWCA | 45 | Lázaro et al., 2009 |
COIR (R) | CCWGTYARMCCHCCWAYAGTAAA | 45 | Lázaro et al., 2009 |
Bar2 (F) | CGTTTAGAGYTNTCTGTTCCAGG | 40 | Lázaro et al., 2009 |
COIbarc_plat_R (R) | TAATTAAAATATAAACCTCAGGATG | 40 | Lázaro et al., 2009 |
BarT | ATGACDGCSCATGGTTTAATAATGAT | 43 | Álvarez-Presas et al., 2011 |
CYB | |||
B3 (F) | TKRTWNTTCAGDTTKTTTCTGG | 44 | This study |
B2 (R) | AAAATAYCACTCNGGCTTWAT | 44 | This study |
N13 | |||
MS13Fi | GGTAGTTGCATAAATTAAAA | 50 | This study |
N13R2 | GCTGAGAAACGGAAGCAAATCGAAGGG | 50 | This study |