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Figure 5 | BMC Evolutionary Biology

Figure 5

From: A bacterial genome in transition - an exceptional enrichment of IS elements but lack of evidence for recent transposition in the symbiont Amoebophilus asiaticus

Figure 5

Contiguous transcription of A. asiaticus IS elements with their downstream genes. Transcription was analyzed with reverse transcriptase PCR. Whole RNA from the Acanthamoeba host and A. asiaticus 5a2 was transcribed into cDNA and subsequently used for PCR. Genomic organization of the tested loci and the expected sizes of the PCR products are shown in Figure 4. Locus_tags are indicated above the gel images; lanes 1: cDNA; lanes 2: positive control, genomic DNA; lanes 3: negative control, no nucleic acids added. Contiguous transcription was demonstrated for all tested loci except for the IS element Aasi_0897 (ISCaa16) and its downstream gene Aasi_1745. The genes Aasi_1200/1201 were used as negative control (as they are located on different DNA strands and have opposing orientation). The groEL/groES operon (Aasi_0308/0309) was used as a positive control. All experiments were performed in three biological independent replicates.

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BMC Ecology and Evolution

ISSN: 2730-7182

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