Figure 5From: Evolutionary view of acyl-CoA diacylglycerol acyltransferase (DGAT), a key enzyme in neutral lipid biosynthesisEvaluation of triacylglyceride biosynthesis in yeast mutants complemented with castor bean DGAT1 or DGT2. The H1122 strain is an are1 are2 double mutant, while H1246 is an are1 are2 Iro1 dga1 quadruple mutant. Lipids were separated in a silica-gel plate of thin-layer chromatography, and the separation range corresponding to Triacylglicerol (TAG) and ADAG (mono-alkyl-diacylglicerol), used as lipid standards, is shown after Coomassie-Blue R250 staining.Back to article page