Figure 5

Relationship between Plasmodium genus conservation score and amino acid variation in P. falciparum drug-resistance regions. For each of three drug-resistance regions, two comparisons between resistant and sensitive isolates were made. (a, b) CRT resistance locus on chromosome 7. (c, d) DHFR resistance locus on chromosome 4. (e, f) DHPS locus on chromosome 8. Mutations in known drug-resistance genes are shown as filled circles; other mutations are shown as open circles. A threshold conservation score of 2.5 is indicated by the dotted line. Only coding regions are displayed, with gene designations and colours used for orientation purposes only. (g) Proportion of total sequence, amino acid variation in drug-resistance genes and amino acid variation in other genes according to conservation level (based on a sliding window of 9 residues, with conservation scores shown within bins of 1.0).