Table 2 Primers used for PCR amplification and sequencing in this study
Target region | Primer sequence (5'-3') | |
|---|---|---|
Adh | Long partial region | Adh1-F1:ATGTCTAATACTGCTGGTCA Adh1-F2: TGATGTTTACTTCTGGGAGG Adh1-R1: GATTGAATTGTGTTCTTTA Adh1-R2: TGTGGATTATGCAACGAAGA Adh2-F: TGTTGACTTCTGGGATGCCAA Adh2-R: ATGCTAACGATGCACCGCAA |
| Â | Internal primer | Adh1-F5: AGGAGAATGCAAGGACTGCGCT Adh2-F5: CATTGCAAGTCTGAGGAAAG |
| Â | reduced Adh1 | Adh1-F2 Adh1-R3: CAAAATGGTAGATAGGCTT |
| Â | Specific primers | spAdh1-F: TCTACCATTTTGTTGGGACT spAdh1-R: AACGCTTCCTGTACATTCAA |
| Â | Â | spAdh2-F: GATTAATCACTTCCTCGGCA spAdh2-R: TAATATAGCCGGTGCACTCT |
LEAFY | Long partial | LFY-F: TGTCGGAGGAGCCAGTGCAA LFY-R: GGCGTAGCAGTGCACATAGT |
| Â | LFY1int2 | LFY1-F: TGGACGTTCATCAATAAAGA LFY1-R: AGTCGAACTAAATAGTTGAA |
| Â | LFY2int2 | LFY2-F: GTGGGCCCATTTCCTGTAGT LFY2-R: GTTAAATCCGGTCAGATTAT |
| Â | LFY2s | LFY2S-F: CTGTATTGACTATTTCTGTC |
| Â | Â | MLFY2-F: CGTACGCTTATTTCTACTGCA LFY2-R |
Actin | Partial | Pact-F: CCATCCAGGCTGTTCTCTC Pact-R: GCAAGGTCCAGACGAAGG |