Asymmetric reproductive isolation between terminal forms of the salamander ring species Ensatina eschscholtzii revealed by fine-scale genetic analysis of a hybrid zone

Table 1 PCR annealing temperatures, sequence length, and primer sequences

Locus	Annealing temp.	Length/ # variable sites (bp)	PCR primer	Primer sequence (5'-3')
ND4	48°C	RFLP	ND4	CACCTATGACTACCAAAAGCTCATGTAGAAGC
			LEU	ACCACGTTTAGGTTCATTTTCATTAC
CXCR4	55°C	116/4	CXCR4F2	TGGTCTGTGGATGCTGTCAT
			CXCR4R	TGCAGTAGCAGATCAAGATGA
SLC8A3	58°C	342/6	SLC8A3F	CATTCGGGTCTGGAATGAAA
			SLC8A3R	ACACCACCATCCCCTCTGTA
RAG1	56°C	359/7	Amp F1	ACAGGATATGATGARAAGCTTGT
			RAG1R	TTRGATGTGTAGAGCCAGTGGTGYTT

ISSN: 2730-7182