Skip to main content
Figure 4 | BMC Evolutionary Biology

Figure 4

From: Why small males have big sperm: dimorphic squid sperm linked to alternative mating behaviours

Figure 4

Determination of sperm number within a single spermatophore. (a) Spermatophores from consort and sneaker males. (b) The relative amount of sperm DNA defined as the propidium iodide fluorescence intensity by flow cytometric analyses from consort (n = 10) and sneaker (n = 9) males. (c) A standard calibration curve was generated by plotting the fluorescence intensity versus sperm concentrations. (d) Estimated sperm number per spermatophore of consort (n = 4) and sneaker (n = 5) individuals. Each bar represents the mean ± s.e.m. of five spermatophores from an individual after the weighted calibration from (b) and (c).

Back to article page