Prey | Empty Vector | MUPP1 (PDZ 10-13) | LNX1 (NPAY-PDZ4) | LNX1 (RING-PDZ2) | LNX2 | ERBB2IP (PDZ) | Nos1 (PDZ) | Dlg2 |
---|
Bait | His3 | β-gal | His3 | β-gal | His3 | β-gal | His3 | β-gal | His3 | β-gal | His3 | β-gal | His3 | β-gal | His3 | β-gal |
---|
TAPP1
| - | - | +++ | +++ | - | - | +++ | +++ | - | - | - | - | - | - | - | - |
SynGAP1
| - | - | +++ | +++ | ++ | - | +++ | +++ | +++ | +++ | - | - | - | - | +++ | +++ |
EphA7
| - | - | +++ | ++ | +++ | + | +++ | +++ | +++ | +++ | - | - | - | - | - | - |
HTR2C
| - | - | +++ | +++ | - | - | - | - | ++ | - | n/d | n/d | n/d | n/d | n/d | n/d |
- The ability of carboxyl terminal sequences from the indicated MUPP1 interacting proteins (baits) to interact with LNX1 and LNX2 (preys) was tested using the yeast two-hybrid system. An empty prey vector and a MUPP1 prey were included as negative and positive controls respectively. Interactions with unrelated PDZ domain-containing proteins (ERBB2IP, Nos1 and Dlg2) were assessed to demonstrate the specificity of binding to LNX proteins. Interactions between bait and prey were indicated by expression of two reporter genes - His3 and LacZ coding for β-galactosidase (β-gal). Truncated LNX1 constructs were used because the full-length protein was not functional in the yeast two-hybrid system. n/d = not determined.