Figure 7

Oikopleura orthologs of Ciona notochord genes expressed in tissues other than the notochord. (A-I) Oikopleura embryos hybridized in situ with the antisense probes indicated at the bottom right of each row. The developmental stages are indicated in the column headings; all embryos are oriented with anterior to the left and dorsal up. The inset in (I) shows a magnified view of a cell specifically stained by Od-tensin, likely part of the caudal ganglion. (J-O) High-magnification views of Oikopleura embryos at 7-8 and 10-12 hpf, hybridized in situ with antisense probes for Od-ACL (J,K), Od-ASAK (L,M) and Od-CaMK (N,O). Black arrows in (J,K) indicate tail neurons (magnified in the insets); yellow arrowheads indicate spiracles; a thin black arrow in (L) indicates putative cells of the base of the Langerhans receptor; green arrowheads indicate the ciliary funnel (M-O) and a black arrow in (O) indicates signal detected in the cerebral ganglion, at the level of the statocyst.