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Table 3 Oligonucleotide primer used to amplify and to sequence partial ERV genes.

From: Evolution of endogenous retroviruses in the Suidae: evidence for different viral subpopulations in African and Eurasian host species

Primer name

Primer sequences (5' → 3')

Expected size (bp)

gag

CTGTTGTTGAAGCGAAAG

1,080

 

TACCTTCAGCCGTGTTG

 

pol

ACCCGCTAACCAAAGA

1,578

 

TGTCTGACCCGATTACC

 

env-A

CCCGAACTCCCATAAACC

1,720

 

AAGGCCCAACTGTAAGTAACA*

 

env-B

CTGCGGCCTGACATAAC

1,060

 

AAGGCCCAACTGTAAGTAACA*

 

env-C

GAACCTGGTGGCCTGATCTAT

1,524

 

GGCCCAACTGTGAGTAACA

 

env-E

ACCTCTTTGCCTGACAATACA

1,320

 

TACAAGGCAGGGAACAAGTAG

 

pol-intF1

CAGACATACCGCTGACTG

n/a

pol-intR1

TGTACTGTCATCCGGGTTCTG

n/a

env-A-intF1

TGGTATGTCTTGGGGAAT

n/a

  1. * The same reverse primer was used to amplify partial fragments of both env classes A and B. Forward primers were specific for each type of env gene.
  2. 1. Internal primers used only for sequencing.
  3. n/a = not applicable
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BMC Ecology and Evolution

ISSN: 2730-7182

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