Figure 6

PARE analysis of the A. thaliana MIR159/319 precursor processing. A: Distribution of uncapped 5'RNA end sequencing frequency (vertical axis) in an approximately 1 kb region (horizontal axis) with the precursor stem-loops located at the centre (green for MIR159; orange for MIR319). The major cleavage sites downstream of the ath-MIR319a stem-loop coincide with a reported alternative splicing site [21]. B: Detailed PARE-detected cleavage within the MIR159/319 precursor stem-loops. Cleavage sites are indicated by arrows tethered with sequencing abundances supporting the cleavages. The most frequently cleaved site for each MIR is indicated by a red asterisk. The sequences corresponding to the four partitions are colored in the same way as in Figure 2.