Figure 3
Expression patterns in Dactylorhiza allotetraploids and their diploid parental species. A. Principal coordinates analysis (PCoA; goodness of fit 0.95 at P = 0.001) of the Dice similarity matrix [54] between allotetraploid Dactylorhiza individuals (filled symbols) and representatives of their diploid parents (unfilled symbols), based on cDNA-AFLP phenotypes. Black-filled symbols, D. majalis s.s.; light grey, D. traunsteineri s.l.; dark grey, D. ebudensis; crosses, D. incarnata; stars, D. fuchsii. For allopolyploids, shapes of symbols and lines denote geographic provenance of samples: dotted line and inverted triangles, Alps; thick line and circles, Scandinavia; thin line and triangles, Pyrenees; dashed line, Britain with rhombus and squares indicating samples from Yorkshire and Scotland, respectively. The three ordination factors together explain 68% of the total variation present in the cDNA-AFLP data matrix. B. Neighbor-joining (NJ) dendrogram based on Nei & Li distances [55] among cDNA-AFLP phenotypes of allopolyploid Dactylorhiza individuals. Numbers above branches are NJ bootstrap percentages (1000 replicates) higher than 70. Symbols follow Figure 3A.