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Table 2 Selectivity filters and NPA-boxes

From: Algal MIPs, high diversity and conserved motifs

   Ar/R selectivity filter a NPA motifs  
Subfamily Proteinb H2 H5 LE1 LE2 Loop B Loop E Substrate specificity
MIPA CcMIPA1;1 H M M R NPM NPA  
  At TIP2;1 H I G R NPA NPA water [58], urea [59], ammonia [60]
MIPB OlMIPB1;1 Y L G R NPS NAA  
  OrMIPB1;1 Y F G R NPS NAA  
  GLA Llac Y V P R NPA NPA water, glycerol [25]
MIPC MpMIPC1;1 L C G V NPT NPA  
  MrMIPC1;1 I T G V NPT NPA  
  OtMIPC1;1 V M C P NPV NPS  
  Zm SIP2;1 c S H G S NPL NPA none [61]
  Os NIP2;1 c G S G R NPA NPA silicic acid [62], arsenite, antimonite [63]
MIPD VcMIPD1;1 N A A R NPA NPA  
  CcMIPD1;1 N A A R NPA NPA  
  CnMIPD1;1 N A A R NPA NPA  
  Cr MIPD1;1 N A A R NPA NPA glycerol [15]
  VcMIPD2;1 T L S R NPA NPA  
  CrMIPD2;1 T L S R NPL NPA  
  CcMIPD3;1 S T A R NPA NPA  
  VcMIPD4;1 N A T H NPT NPA  
  Os TIP4;1 T T A R NPA NPA water, glycerol [29]
MIPE OrMIPE1;1 F H C R NPA NPA  
  CnMIPE1;1 F H C R NPA NPA  
  CnMIPE1;2 A H C R NAA NPT  
  CnMIPE1;3 F H C R NPA NPA  
  Hs AQP5 F H C R NPA NPA water [34]
PIP CcPIP4;1 F H C R NPA NPA  
  CcPIP4;2 F H C R NPA NPA  
  At PIP2;3 F H T R NPA NPA water [64]
GIP PbcvMT325GIP1;1 F V I R NPA NPA  
  CcGIP1;1 F L N R NPA NPA  
  CnGIP1;1 F I I R NPA NPA  
  Pp GIP1;1 F V P R NPA NPA glycerol [24]
  1. a) The ar/R filter is defined by four amino acid residues: one in helix 2, one in helix 5 and two in loop E b) Protein names written in bold are MIPs with studied substrate specificities used as reference. They are chosen based on the similarity of their ar/R filter to the ar/R filters of the members within the group c)The MIPCs have very diverse ar/R filters, making it hard to find a reference. Therefore two MIPs with ar/R filters somewhat similar to those of the MIPCs are displayed.