Expression of slc11a2-α and slc11a2-β in sea bass leukocytes under experimental (a) iron overload or (b) infection, in vitro. Samples were collected at 0, 6, 12, 24, 48 and 72 hours after addition of ferric ammonium citrate (iron overload) or heat-inactivated P. damselae (infection). Values are expressed as mean fold change ± S.D. (n = 5). β-actin was used as the housekeeping gene. Differences from the control group were considered significant at *p < 0.05.