Estimation of divergence time between two sibling species of the Anopheles (Kerteszia) cruziicomplex using a multilocus approach

Table 3 Genetic differentiation between Florianópolis and Itaparica.

Locus	F _ST	P (F_ST)	Dxy	Da	S _s	S _f	S ₁	S ₂
*timeless*	0.8150 (0.8144)	0.0000 (0.0000)	0.1154 (0.0877)	0.0941 (0.0714)	07 (01)	25 (15)	56 (17)	16 (12)
*Clock*	0.7088 (0.7500)	0.0000 (0.0000)	0.0593 (0.0579)	0.0420 (0.0434)	00 (00)	03 (04)	07 (06)	08 (06)
*cycle*	0.5806 (0.5852)	0.0000 (0.0000)	0.0371 (0.0441)	0.0215 (0.0258)	01 (00)	02 (02)	21 (13)	02 (03)
*Rp49*	0.8854 (0.8903)	0.0000 (0.0000)	0.0606 (0.0695)	0.0536 (0.0619)	00 (00)	12 (12)	10 (09)	09 (09)
*RpS2*	0.8502 (0.8598)	0.0000 (0.0000)	0.0843 (0.0836)	0.0717 (0.0718)	00 (00)	16 (16)	18 (18)	08 (07)
*RpS29*	0.8865 (0.9276)	0.0000 (0.0000)	0.0843 (0.0950)	0.0747 (0.0881)	00 (00)	11 (19)	14 (05)	19 (11)

F_ST, pair-wise estimates of population differentiation. P-value, significance of F_STvalues (evaluated by 1,000 random permutations). Dxy and Da, average number of nucleotide substitutions per site and the number of net nucleotide substitutions per site between species, respectively [38]. S₁, number of polymorphic sites exclusive to Florianópolis. S₂, number of polymorphic sites exclusive to Itaparica. S_sand S_f, number of shared polymorphisms and number of fixed differences between the two species, respectively. Numbers in parentheses are related to the non-recombining block (NR) for each locus.

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