Figure 6

Embryonic expression patterns of Sp genes in Thermobia domestica. (a-c) Expression of the Sp1-4 representative at the early germ band stage (a), mid germ band elongation (b), and late germ band elongation (a). (a') is the epifluorescence image (Sytox Green staining) of the embryo in a. (d-h) Expression of the Sp5/btd representative at the blastoderm stage (d), early germ band stage (e), starting germ band elongation (f), early germ band elongation (g), and late germ band elongation (h). The arrow in d-f points to the early head stripe. The arrowheads in f, h point to metameric stripes. The arrow in h points to expression in ventral nervous system. (d') is the epifluorescence image (Sytox Green staining) of the embryo in d. (i-l) Expression of the Sp6-8 representative at mid germ band elongation (i, j; the embryo in j is slightly more advanced than the one in i), early germ band retraction (k), and late germ band retraction (l). The arrow in i-k points to segmental stripes in young segments that just have formed from the posterior growth zone. All embryos are oriented with anterior to the top. Abbreviations see Fig. 4.