Genetic population structure of sympatric and allopatric populations of Baltic ciscoes (Coregonus albulacomplex, Teleostei, Coregonidae)

Table 3 Primers for the selective amplification step of AFLP

Primer combination	MseI Primer	EcoRI A	EcoRI B	Number of loci per primer pair
1	CAT	ACTfam	AAGjoe	208
2	CAG	ACTfam	AAGjoe	209
3	CTA	ACTfam	ACAjoe	214

All primers had 3 additional bases on the 3' end. EcoRI primers A and B were labelled with different fluorescent dyes (fam and joe) and 3 different multiplex reactions were run with two forward and one backward primer each.

ISSN: 2730-7182