Table 4 Long-term heat treatment protocol. Those rounds with an "x" for the Replating column signify that the entire treatment tube was plated on E. coli C and incubated overnight at 37°C.
From: Adaptive evolution and inherent tolerance to extreme thermal environments
Selection round | Temperature (°C) | Duration (hours) | Replating |
|---|---|---|---|
1A (Line A) | 70 | 24 | |
1B (Line B) | 70 | 48 | |
2 | 50 | 24 | x |
3 | 75 | 72 | x |
4 | 70 | 24 | x |
5 | 50 | 65 | |
6 | 50 | 24 | x |
7 | 50 | 96 | x |
8 | 50 | 24 | |
9 | 50 | 72 | x |