Figure 3

Experimental design for long-term treatment of phage lysate to elevated temperatures. Each virus lysate tube was submerged in water and heated, after which it was either entirely plated with E. coli C or an aliquot was plated and the remaining lysate was used for subsequent heating. In the case of the former, the plate was harvested after overnight incubation and used for subsequent treatments (as indicated in the figure by the gray dashed line).