Microevolutionary dynamics of a macroevolutionary key innovation in a Lepidopteran herbivore

Table 4 Estimates of population differentiation.

Analysis	pop	PraNSP-D2	PraNSP-D3	IDH	Ga3pdh	COI	ArgKinase	Wingless
	DE-FR	0.03	-0,02*	-0.01	0.14*	-0.05	-0.01	0.00
	DE-IT	0.00	0.02	0.03	0.05	-0.03	0.01	0.01
Fst	DE-US	0.01	0,09*	-0.02	0.04	0.20*	-0.04	0.00
	FR-IT	0.03	0.04	0.10	0.29**	0.00	0.01	0.03
	FR-US	0.06	0.13	-0.04	0.17*	0.14	0.04	0.00
	IT-US	0.03	0.07	0.07	0.02	0.11	0.05	0.02
	DE-FR	0.0009*	0.0082*	0.4361	0.0279	0.4868	0.0867	0.7204
	DE-IT	0.0321	0.1859	0.3974	0.1286	0.5966	0.4368	0.3702
Exact Test	DE-US	0.0373	0.0001*	0.9271	0.0177	0.0507	0.6965	0.1252
	FR-IT	0.1009	0.0911	0.0060	0.0022*	0.1747	0.6628	0.4554
	FR-US	0.0007*	0.1322	0.7671	0.0525	0.3480	0.1460	0.2003
	IT-US	0.0039	0.1107	0.0842	0.5326	0.2481	0.2489	0.0310
	DE-FR	15.48	inf	inf	3.01	inf	inf	128.35
	DE-IT	101.85	30.21	14.32	10.31	inf	90.30	35.16
Migration	DE-US	45.25	4.92	inf	12.93	1.93	inf	inf
	FR-IT	16.88	11.76	4.65	1.22	inf	33.18	16.44
	FR-US	8.20	3.33	inf	2.40	3.04	10.70	inf
	IT-US	15.79	6.49	6.50	32.73	4.23	10.06	20.90

Fst values, p-values for the exact test and the estimated absolute number of migrants between two populations M as determined in the Arlequin program are given for every population comparison for every sequenced gene. For easier comprehension high migration values (above 30) are in bold. Analysis always includes the whole sequenced fragments, thus both exons and intron in Pra Nsp-D2 and -D3. For both the Fst and Exact Tests, P-values significant after Bonferroni corrections (< 0.05) within gene are marked with an asterisk. If the p-value is < 0.001 (after Bonferroni) two asterisks are used.

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