Figure 1

PCR-RFLP analysis of make-up of the ITS region in the hybrid with restriction enzyme Hae III at early development. Panel A exhibits PCR-RFLP analysis at the 2-cell stage (about 1 hour after fertilization) with samples from maternal parent C. farreri (Maternal), paternal parent A. irradians (Paternal), and samples from five random selected hybrid individuals (1 through 5), and molecular weight standard (MW). Panel B exhibits PCR-RFLP analysis at the trochophore stage (about 20 hour after fertilization) with samples from six random selected hybrid samples (1 through 6), and molecular weight standard (MW). Panel C exhibits PCR-RFLP analysis at the early umbo larva stage (about 4 days after fertilization) with samples from six random selected hybrid samples (1 through 6), and molecular weight standard (MW). Panel D exhibits PCR-RFLP analysis at the middle umbo larva stage (about 10 days after fertilization) with samples from six random selected hybrid individuals (1 through 6), and molecular weight standard (MW). Panel E exhibits PCR-RFLP analysis at the late umbo larva stage (about 14 days after fertilization) with samples from maternal parent (Maternal), paternal parent (Paternal), and samples from four random selected hybrid individuals (1 through 4) and a sample from mixed samples of multiple individuals (5*), and molecular weight standard (MW). Molecular weight standards were 100 bp DNA ladder.