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Figure 8 | BMC Evolutionary Biology

Figure 8

From: Retrotransposition and mutation events yield Rap1 GTPases with differential signalling capacity

Figure 8

Analysis of the effect of mRap1A-retro1, -retro2 and hRa1pB-retro on cell spreading. A. A431 cells were co-transfected with histon2B-GFP and Rap1 constructs as indicated. Cells were grown overnight in tissue culture wells and then photographed. GFP-positive cells were visualized by phalloidin (red) staining. B. For cell spreading, first we determined the average surface of >100 non-transfected cells using the ImageJ program. Next, the surface of at least 90 transfected cells (selected on the basis of the presence of GFP-H2B) from two independent experiments was measured. Cells with a surface area of more than twice that of untransfected cells were scored as spread. ** indicates a p-value < 0.001 (Chi-test).

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