Figure 7
From: Retrotransposition and mutation events yield Rap1 GTPases with differential signalling capacity
Analysis of the effect of mRap1A-retro1, -retro2 and hRap1B-retro on cell adhesion. Jurkat cells stably expressing Epac (HB6 cells) were co-transfected with TK luciferase reporter plasmid and either empty vector (EV) or Rap1 constructs as indicated. After 42 h cells were replated and seeded in triplicate wells coated with fibronectin and after 1 h the percentage of adherent cells was measured. Results are representative of three independent experiments. The expression of HA-Rap and HA-RalGDS-RBD was determined by Western blotting using anti-HA as a probe (data not shown).