Figure 2
From: Retrotransposition and mutation events yield Rap1 GTPases with differential signalling capacity
RT PCR reaction of mRap1A and mRap1A-retro1, mRap1A-retro2 mRNA. A. RT-PCR reaction with RNA isolated from WT organs. One half of RT-PCR reaction mixture was directly applied on gel (lines 1-9) while the second half was first digested with NlaIV (lines 10-18). B. RT-PCR product was digested with NlaIV restriction enzyme and loaded on the gel. Lanes 1 to 9 represent samples from wt organs (+/+) and lanes 10 to 18 represent organs of Rap1A deficient mice (-/-).