Two Frequenins in Drosophila: unveiling the evolutionary history of an unusual Neuronal Calcium Sensor (NCS) duplication

Table 1 Functional divergence between NCS subfamilies

Subfamily (Class)	Subfamily (Class)	Type I θ (SE)	Type II θ (SE)	Positions^a
Frq/NCS-1 (A)	Recoverin (C)	0.246 (0.089)	0.342 (0.072)	79, 102, 162
	Hippocalcin (B)	0.858 (0.230)	0.273 (0.046)	79, 102, 162
	KChIP1 (E)	0.899 (0.233)	0.415 (0.063)	58, 79, 102, 138
	KChIP2 (E)	NS	0.348 (0.078)	58
	KChIP3 (E)	0.594 (0.268)	NS	138, 162
	KChIP4 (E)	0.561 (0.232)	0.447 (0.057)	58, 138, 162
	VILIP2 (B)	NS	0.298 (0.052)	102
	VILIP3 (B)	0.829 (0.285)	-	79, 102
Recoverin (C)	VILIP2 (B)	NS	0.317 (0.076)	102
	VILIP3 (B)	0.563 (0.187)	NS	102
	GCAP1 (D)	0.665 (0.069)	0.480 (0.088)	79, 161
	GCAP2 (D)	0.765 (0.076)	0.501 (0.077)	161, 161
	KChIP1 (E)	0.778 (0.090)	NS	91
	KChIP2 (E)	0.867 (0.095)	NS	79
	KChIP3 (E)	0.665 (0.070)	0.547 (0.074)	79, 161
	KChIP4 (E)	0.731 (0.100)	0.500 (0.070)	79, 161, 162
Hippocalcin (B)	KChIP1 (E)	NS	0.455 (0.060)	58
	KChIP2 (E)	NS	0.430 (0.073)	58
	KChIP4 (E)	NS	0.496 (0.055)	58, 162
VILIP2 (B)	KChIP1 (E)	NS	0.483 (0.060)	161
	KChIP2 (E)	NS	0.517 (0.066)	161
	KChIP3 (E)	NS	0.416 (0.076)	161
	KChIP4 (E)	NS	0.546 (0.055)	161
GCAP3 (D)	GCAP2 (D)	NS	0.498 (0.127)	79

^a Sites with changes between Frq1 and Frq2 that show significant shift in their evolutionary rate between NCS subfamilies (type I and type II sites are in regular and italic font, respectively). Positions are numbered relative to the D. melanogaster Frq1 protein. θ, ML estimate of the coefficient of functional divergence. SE, standard error. NS, non-significant.

ISSN: 2730-7182