Table 2 Comparison of the relative sugar binding activities of Con-anc mutants, Con-anc-N/C/L5, Con-anc-N/C/L5/L6, and Con-anc-N/C/L5/L6/L3.
Compared sugars | Ratio of relative binding activities | Different structure(s) between two compared sugars | ||
|---|---|---|---|---|
| Â | Con-anc N/C/L5 | Con-anc N/C/L5/L6 | Con-anc N/C/L5/L6/L3 | Â |
#45/#41 | 2.48 | 2.48 | 0.9 | α1,3- fucosyl(GlcNAc) |
#43/#42 | 1.01 | 1.11 | 1.29 | α1,2- fucosyl(Gal) |
#44/#42 | 6.18 | 5.27 | 1.89 | α1,4-fucosyl(GlcNAc) |
#46/#42 | 10.7 | 8.76 | 2.02 | α1,2- fucosyl(Gal)/α1,4- fucosyl(GlcNAc) |
#47/#42 | 1.62 | 1.55 | 1.63 | α1,2- fucosyl(Gal)/α1,3- GalNAc(Gal) |
#48/#42 | 16.2 | 11.4 | 2.71 | α1,4-fucosyl(GlcNAc)/α1,2- fucosyl(Gal)/α1,3-GalNAc(Gal) |
#46/#43 | 10.6 | 7.91 | 1.57 | α1,4-fucosyl(GlcNAc) |
#46/#44 | 1.73 | 1.66 | 1.07 | α1,2- fucosyl(Gal) |
#47/#43 | 1.61 | 1.4 | 1.27 | α1,2- fucosyl(Gal) |
#48/#43 | 16.1 | 10.3 | 2.1 | α1,4-fucosyl(GlcNAc)/α1,3- GalNAc(Gal) |
#48/#46 | 1.52 | 1.3 | 1.34 | α1,3- GalNAc(Gal) |
#48/#47 | 10 | 7.34 | 1.66 | α 1,4-fucosyl(GlcNAc) |