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Table 1 Comparison of the dissociation constants (Kd) of ConI, ConII, Con-anc, and Con-anc mutants.

From: Protein engineering of conger eel galectins by tracing of molecular evolution using probable ancestral mutants

Sugar #

ConI

ConII

Con-anc

Con-anc-N/C

Con-anc-L5

Con-anc-N/C/L5

 

Kd (μM)

ratio to Con-anc.

ratio to ConI

Kd (μM)

ratio to Con-anc.

ratio to ConI

Kd (μM)

ratio to Con-anc.

ratio to ConI

Kd (μM)

ratio to Con-anc.

ratio to ConI

Kd (μM)

ratio to Con-anc.

ratio to ConI

Kd (μM)

ratio to Con-anc.

ratio to ConI

01

0.50

10

1

3.8

1.4

0.13

5.2

1

0.10

13

0.41

0.04

3.2

1.6

0.16

1.7

3.1

0.30

02

0.36

7

1

2.3

1.0

0.15

2.4

1

0.15

7.1

0.34

0.05

2.0

1.2

0.18

1.2

1.9

0.29

03

0.24

7

1

1.4

1.1

0.17

1.6

1

0.15

4.9

0.33

0.05

1.3

1.2

0.18

0.78

2.1

0.31

04

0.36

7

1

2.3

1.1

0.16

2.5

1

0.15

7.6

0.33

0.05

1.9

1.3

0.19

1.3

2.0

0.29

05

0.58

8

1

4.0

1.1

0.14

4.6

1

0.13

14

0.32

0.04

3.2

1.4

0.18

2.0

2.3

0.29

06

0.51

8

1

3.4

1.2

0.15

4.0

1

0.13

12

0.35

0.04

2.6

1.6

0.20

1.8

2.2

0.28

09

0.51

9

1

3.8

1.2

0.13

4.7

1

0.11

14

0.34

0.04

3.0

1.6

0.17

1.9

2.4

0.26

10

0.42

6

1

2.5

1.1

0.17

2.7

1

0.16

8.0

0.33

0.05

2.0

1.3

0.20

1.3

2.0

0.31

11

0.37

7

1

2.3

1.1

0.16

2.5

1

0.15

7.3

0.34

0.05

2.0

1.3

0.19

1.3

1.9

0.28

21

1.2

10

1

9.6

1.3

0.13

13

1

0.10

33

0.39

0.04

6.7

1.9

0.18

4.4

2.9

0.28

22

1.4

11

1

12

1.3

0.12

15

1

0.09

46

0.32

0.03

7.8

1.9

0.18

4.2

3.5

0.32

23

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

41

0.96

8

1

9.4

0.8

0.10

7.7

1

0.12

23

0.34

0.04

3.5

2.2

0.28

2.2

3.5

0.43

42

0.31

8

1

2.5

1.0

0.12

2.6

1

0.12

6.5

0.40

0.05

1.3

2.0

0.24

0.74

3.5

0.42

43

0.35

6

1

2.5

0.8

0.14

2.0

1

0.18

5.5

0.36

0.06

1.4

1.4

0.26

0.83

2.4

0.42

26

6.8

4

1

28

1.0

0.25

27

1

0.26

69

0.39

0.10

18

1.5

0.37

12

2.2

0.55

27

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

28

2.8

17

1

26

1.8

0.11

46

1

0.06

112

0.41

0.02

20

2.2

0.13

11

4.2

0.25

29

-

-

-

51

4.7

-

242

1

-

332

0.73

-

168

1.4

-

215

1.1

-

30

-

-

-

66

3.0

-

196

1

-

364

0.54

-

190

1.0

-

696

0.3

-

31

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

32

4.5

8

1

18

2.0

0.25

37

1

0.12

74

0.50

0.06

22

1.7

0.21

14

2.6

0.32

33

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

34

6.4

8

1

25

1.9

0.26

49

1

0.13

104

0.47

0.06

27

1.8

0.24

17

2.8

0.37

38

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

39

2.3

24

1

31

1.7

0.07

54

1

0.04

116

0.47

0.02

20

2.8

0.12

8.1

6.7

0.28

40

-

-

-

75

0.8

-

63

1

-

178

-

-

136

-

-

94

-

-

44

4.4

13

1

24

2.3

0.18

55

1

0.08

126

0.44

0.04

3.9

14

1.14

1.7

32

2.6

45

13

13

1

122

1.4

0.11

171

1

0.08

550

0.31

0.02

24

7.3

0.55

12

15

1.1

46

9.4

5

1

37

1.2

0.26

44

1

0.21

183

0.24

0.05

4.5

9.8

2.10

2.1

21

4.5

47

3.8

9

1

43

0.8

0.09

34

1

0.11

115

0.30

0.03

12

2.7

0.31

5.6

6.1

0.69

48

17

3

1

42

1.3

0.40

56

1

0.30

166

0.34

0.10

5.6

9.9

2.99

2.5

22

6.6

49

10

6

1

44

1.4

0.23

62

1

0.16

158

0.39

0.06

37

1.7

0.27

19

3.3

0.53

50

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

Sugar #

Con-anc-N/C/L5/L6

Con-anc-N/C/L5/L6/L3

            
 

K d (μM)

ratio to Con-anc.

ratio to ConI

K d (μM)

ratio to Con-anc.

ratio to ConI

            

01

1.0

5.0

0.47

0.70

7.4

0.71

            

02

0.77

3.1

0.46

0.49

4.9

0.73

            

03

0.55

2.9

0.44

0.39

4.1

0.62

            

04

0.75

3.3

0.48

0.49

5.1

0.74

            

05

1.2

3.9

0.50

0.80

5.7

0.73

            

06

1.1

3.6

0.46

0.73

5.5

0.70

            

09

1.2

3.9

0.43

0.74

6.4

0.69

            

10

0.86

3.1

0.48

0.61

4.4

0.68

            

11

0.85

2.9

0.44

0.51

4.8

0.72

            

21

2.5

5.0

0.49

1.7

7.5

0.73

            

22

2.7

5.4

0.51

1.9

7.6

0.71

            

23

-

-

-

-

-

-

            

41

1.3

5.9

0.73

1.0

7.7

0.96

            

42

0.52

4.9

0.59

0.40

6.4

0.76

            

43

0.53

3.7

0.66

0.35

5.5

0.99

            

26

6.2

4.3

1.1

4.8

5.5

1.42

            

27

-

-

-

-

-

-

            

28

6.1

7.6

0.46

5.2

8.8

0.53

            

29

-

-

-

-

-

-

            

30

-

-

-

-

-

-

            

31

-

-

-

-

-

-

            

32

12

3.1

0.38

7

5.6

0.68

            

33

-

-

-

-

-

-

            

34

13

3.7

0.48

9

5.7

0.75

            

38

-

-

-

-

-

-

            

39

3.9

14

0.58

4

15

0.64

            

40

28

-

-

238

0.27

-

            

44

1.4

39

3.1

3.0

19

1.49

            

45

7.1

24

1.8

15

11

0.87

            

46

1.8

24

5.1

6.0

7.3

1.57

            

47

4.1

8.3

0.94

3.0

11

1.30

            

48

2.5

22

6.7

8.1

6.9

2.08

            

49

11

5.9

0.95

8.7

7.2

1.16

            

50

-

-

-

-

              
  1. Ratios of Ka (1/Kd) values of congerins and their mutants to those of Con-anc and ConI were calculated. PA sugar numbers are provided in Additional File 3: Supplemental Figure S1.
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