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Figure 2 | BMC Evolutionary Biology

Figure 2

From: Incomplete concerted evolution and reproductive isolation at the rDNA locus uncovers nine cryptic species within Anopheles longirostrisfrom Papua New Guinea

Figure 2

ITS2 genotypes of A. longirostris. Panel A: ITS2 PCR products run through a 1.0% agarose gel indicate some small size variation. Panel B: The same ITS2 PCR products cut with Msp I and run through a 3.0% agarose gel reveals eight distinct RFLP genotype profiles. Panel C: Most ITS2-RFLP genotypes revealed the same heteroduplex profile when PCR products were run through a 7.0% acrylamide gel suggesting copy variants are fixed within individuals and within interbreeding populations. However, genotype C contained two distinct heteroduplex profiles (designated C1 and C2) within its RFLP profile revealing the presence of two independently evolving ITS2 genotypes.

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BMC Ecology and Evolution

ISSN: 2730-7182

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