Positive selection at high temperature reduces gene transcription in the bacteriophage ϕX174

Table 2 Transcript level from the D promoter is significantly reduced in promoter mutant strains at any temperature, host or time point*.

Temperature	Host	Time	F-test	Pr > F
37°	Escherichia coli	4 min	395	<0.0001
37°	Escherichia coli	8 min	397	<0.0001
37°	Salmonella typhimurium	4 min	53	<0.0001
37°	Salmonella typhimurium	8 min	209	<0.0001
37°	Shigella sonnei	4 min	450	<0.0001
37°	Shigella sonnei	8 min	1112	<0.0001
42°	Escherichia coli	4 min	143	<0.0001
42°	Escherichia coli	8 min	390	<0.0001
42°	Salmonella typhimurium	4 min	381	<0.0001
42°	Salmonella typhimurium	8 min	473	<0.0001
42°	Shigella sonnei	4 min	94	0.0002
42°	Shigella sonnei	8 min	143	<0.0001

*ANOVA on relative transcript levels for each environmental condition and time point with 1 degree of freedom for each test.

ISSN: 2730-7182