Gene structure, transcripts and calciotropic effects of the PTH family of peptides in Xenopus and chicken

Table 2 Primer pairs (same prefix ending in fw or rv) used to amplify the Xenopus and chicken PTH family members.

	*Xenopus laevis*	Chicken
PTH	PTHfw: aggagacgggctgtgagtgag$	PTHfw: atgacttctacaaaaaatctg$
	PTHrv: tcattggatgccaggcttta$	PTHrv: tggcttagttttaaagagta$
	PTH2fw: tcagatgaagttacaggac*	PTHfw: gcataaccttggagagcatcg*
	PTH2rv: cttagtgctatgcctatg*	PTHrv: cctctgggtcctggcatc*
PTHrP	PTHrPfw: cagtatctccacgacaaagg$*	PTHrP(1-139)fw: ctgagagcccagtcttgga$
	PTHrP(1-131)rv: ttacctgtaatctaattcttcca$	PTHrP(1-139)rv: gggtaacaatttcagtaact$
	PTHrP(1-144)rv: cgggtgccgctcatctgc$	PTHrP(1-141)5utrAfw: gaagggagtagcacctgggc$
	PTHrPrv: tggtggcagggagtaag*	PTHrP(1-141)5utrBfw: ggcacctgcttttaaaaccc$
		PTHrP(1-141)5utrCfw: gctaacagaggaactgcgac$
		PTHrP(1-141)5utrDfw: aggactgacccctcctttcc$
		PTHrP(1-141)rv: gatcccctctactgatcttcc$
		PTHrP(1-139)fw: agcaaagcctggaaaacg*
		PTHrP(1-139rv: gtggaaaagatacagcagaattacc*
		PTHrP(1-141)5utrAfw: caggcttgcggtgaggcta*
		PTHrP(1-141)5utrArv: gcgaaactccactgctgaaag*
		PTHrP(1-141)5utrBfw: tgacccctcctttccttgc*
		PTHrP(1-141)5utrBrv: ggcacagaataactcagaagaaac*
		PTHrP(1-141)5utrCfw: cagaggaactgcgacgaacaac*
		PTHrP(1-141)5utrCrv: gcgaaactccactgctgaaag*
		PTHrP(1-141)5utrDfw: ggcacctgcttttaaaaccc*
		PTHrP(1-141)5utrDrv: aaggttttgatgaaagataggaatcc*
PTH-L	PTH-Lfw: gagagatcagttgcagagg$	PTH-Lfw: gaacgacaagagaaggaaag$
	PTH-Lrv: tgaaggatcccgctccatt$	PTH-Lrv: ctgcttcatcgggtttga$
	PTHLfw: ttgaagaaataaatcgccagag*	PTHLfw: gataaggcgagggcatttcaag*
	PTHLrv: atgctgctgattctttgctgt*	PTHLrv: cctgctgctggctgtgtg*
r18S	18s fw tgacggaagggcaccaccag*	18s rv aatcgctccaccaactaagaacgg*

Note: $ indicates primers for RT-PCR and * indicates primers for q-PCR. For Xenopus PTHrP the same forward primer was used for each pair.

ISSN: 2730-7182