Comparative chromosome painting (CCP) in Pachycladon cheesemanii. Labelling scheme, in situ localization within a pachytene complement and straightened pachytene bivalent for each of the ten chromosomes (PC1-PC10) are shown. Chromosomes were identified by CCP with Arabidopsis BAC clones and contigs labelled by biotin-dUTP (red), digoxigenin-dUTP (green), and Cy3-dUTP (yellow). Due to the duplicated nature of Pachycladon genomes, each painting probe labels two homeologous chromosome regions on different chromosomes (white and yellow acronyms). Chromosomes counterstained by DAPI. NOR: nucleolar organizing region. Scale, 10 μm.