Insights into the role of differential gene expression on the ecological adaptation of the snail Littorina saxatilis

Table 1 Transcripts analyzed by a three-way nested ANOVA that showed significant expression differences between ecotypes.

Transcripts	F _Ecotype	F _Transect	F _{E × T}	F _{Specimen(E × T)}	RB (average ± ES)	SU (average ± ES)	F _{randomization}
1^#	8.93**	0.19	0.12	6.07**	1057 ± 225.9	2317 ± 341.0	9.48**
10	6.87*	0.012	0.24	12.38***	715 ± 161.9	258 ± 43.1	7.41*
29	38.70***	0.62	2.40	0.53	304 ± 94.9	649 ± 100.8	6.20*
41	6.49*	1.53	0.002	1.41	1023 ± 335.6	4381 ± 411.3	39.94***
57	10.71**	0.43	0.11	2.98*	1289 ± 343.1	3038 ± 435.5	11.13***
63	5.57*	1.04	0.23	6.14**	1441 ± 479.3	3388 ± 674.0	5.53*
64	11.01**	1.69	0.07	9.67***	579 ± 144.6	2130 ± 458.6	10.40**
73	5.98*	0.46	0.09	5.77**	652 ± 175.9	1443 ± 264.4	6.20*
76^#	8.13*	0.01	0.05	24.34***	2342 ± 652.7	441 ± 77.9	7.25*
78	7.22*	0.63	0.07	5.52**	1108 ± 230.3	402 ± 156.9	5.82*
85	6.43*	0.01	0.14	3.22*	464 ± 54.1	1605 ± 429.9	6.92**
96	6.15*	2.14	0.87	2.43	391 ± 105.9	1117 ± 303.7	4.47*

*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001
# loci showing significant expression between sexes of the RB ecotype [11].
We represent the F value for the factors ecotype, transect, interaction, and nested considering the biological replicates. In RB and SU, the means± standard errors of the RFU (relative fluorescence units) are shown. The randomization ANOVA (F_{randomization}) was analyzed on the mean trancript intensity across technical replicates. Underlined probabilities were still significant after multitest correction by SGoF [67].

ISSN: 2730-7182