Figure 2
From: Pseudogenes as an alternative source of natural antisense transcripts
Fraction of 77 pseudogenes (putatively expressing an EST in anti-sense) that have a mutation respect to the homologous position in the parental gene at a given position in their sequence. Blue curve: distance is taken upstream the putative PAS of the EST +10 nt (the PAS is at position × = 10); the curve indicates that there is a region of high conservation in a 50 nt region upstream the putative PAS (gray box). Red curve: distance is taken downstream the 5'-end of the EST -100 nt; no region of high conservation is present. Values are averaged in a window of five nucleotides. The difference between the two distributions is statistically significant (p-value = 4.482 × 10^-7 from a Kolmogorov-Smirnov test).